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Fig. 6. Knockdown of Charon leads to a defect in heart laterality. (A) A pharyngula-stage (24 hpf) embryo that received 25 pg of charon RNA. (B) An embryo that received both charon RNA (25 pg) and charon-MO (0.8 ng). (C-K) The charon morphant embryos displayed variable defects in heart positioning. The numbers of embryos showing each phenotype are shown in Table 2. nkx2.5 expression at 26 hpf (C-E), and cardiac myosin light chain (cmlc2) expression at 26 hpf (F-H) and 52 hpf (I-K). At 26 hpf, the heart jogged to the left (C,F), to the right (E,H), or did not jog (D,G) in charon morphant embryos. Likewise, heart looping was disrupted in charon morphant embryos at 52 hpf (D-loop, I; no-loop, J; L-loop, K). (L) The charon morphant embryos showed no gross morphological abnormalities at 40 hpf. (A,B,L) Lateral views. (C-E, I-K) Ventral views. (F-H) Dorsal views.





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