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Fig. 7. Charon is required for the early processes of L/R patterning. (A-I) Embryos
were injected with charon-MO and examined for atv/lefty1 (20
hpf, A-C), spaw (18 hpf, E-F), and pitx2 (20 hpf, G-I)
expression. In addition to the normal (left-sided) expression of the gene
(A,D,G), bilateral (B,E,H) or reversed (C,F,I) expression of the
left-side-specific markers was also observed. The numbers of embryos showing
each phenotype are given in Table
3. Embryos that showed strong right-side stain and weaker
left-side stain were recorded as bilateral (dorsal views). Arrowheads indicate
the presence of the expression. (J-T) Midline barriers are not affected in
charon morphant embryos. no tail (ntl) expression
in notochord of wild-type (J,K) and charon morphant embryos (L,M) at
13 hpf. sonic hedgehog (shh) expression in notochord at 13
hpf (N,O) and in floor plate at 24 hpf (R,S) in wild-type (N,R) and
charon morphant embryos (O,S). antivin/lefty1 expression in
wild-type (P) and charon morphant embryos (Q) at the 6-9-somite
stage. Saggital sections of ntl expression at 24 hpf in wild-type (T)
and charon morphant embryos (U). (J,L,R,S) Lateral views. (K,M,N,O)
Dorsal views. (P,Q) Dorso-lateral views.
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