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Fig. 8. Southpaw is epistatic to Charon. Control (uninjected) embryos (A,D,G), and embryos injected with charon-MO (0.8 ng, B,E,H), or co-injected with charon-MO (0.8 ng) and southpaw-MO (8 ng) (spaw-MO+charon-MO, C,F,I), were examined for pitx2 (22-24-somite stage, A-C), cyclops (cyc) (18-21-somite stage, D-F), and antivin/lefty1 (18-21-somite stage, G-I) expression. (A-C) `Face-on' views, optical sections. Similar effects were seen on pitx2 expression in the lateral plate. Arrowheads indicate pitx2 expression in dorsal diencephalons. (D-F) Dorsal views, with anterior to the top. Arrowheads indicate cyc expression in dorsal diencephalon and lateral plate mesoderm. (G-I) Dorsal views, with anterior to the left. Arrowheads indicate antivin/lefty1 expression in dorsal diencephalon and lateral plate mesoderm. Typical data are shown in this Figure and the numbers showing laterality defects are given in Table 3.





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