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Fig. 8. Dlx3 depletion blocks the otic expression of Sox9. (A) Injection of 30 ng of Dlx3-mo in one animal ventral blastomere at the two-cell stage reduces Sox9 otic expression (injected side, arrow). For comparison, the control side shows the normal expression of Sox9 in the presumptive otic placode (arrow). Pax8 expression was not significantly inhibited by this procedure. Lateral views of stage 14 embryos, anterior towards the right (left panel). (B) Dlx3-mo-injected embryos form normal otic vesicle at stage 35 (arrows). br, brain; no, notochord; ov, otic vesicle.





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