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Fig. 4. Transcriptional control of daf-9 by daf-12. (A,B) An N2 wild-type larva carrying a daf-9p::daf-9 genomic::GFP transgene at late L2 stage. (A) Normaski image. (B) Fluorescence image showing strong daf-9::GFP expression in the hypodermis. (C,D) A dpy-7(sc27) daf-12(m20) larva carrying a daf-9p::daf-9 genomic::GFP transgene at late L2 stage. (C) Normaski image. (D) Fluorescence image showing daf-9::GFP expression in the XXXL/R cell but not in the hypodermis. (E) Summary of daf-9::GFP expression pattern driven by five transgenes that encompass different promoter, exonic and intronic sequence from the endogenous daf-9 locus. In construct v, a nuclear receptor (NR) consensus half site (underlined) in intron 1 was mutated to a LexA-binding site with the altered bases in grey. Transgenes i, ii and iv rescued the dauer arrest phenotype of daf-9(e1406) dpy-7(sc27) mutant animals. n.d., not determined.





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