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Fig. 8. Effects of transient ß-catenin activation in
K14
Nß-cateninER transgenic mice. (A) Schematic representation of
experiments performed with the D2 and D4 transgenic lines. The arrow
represents time in days (not to scale). Dots are time points analysed.
Continuous lines connecting dots represent periods of daily 4OHT treatment and
dashed lines indicate no 4OHT treatment. (B-G) Mice received the number of
doses (d) of 4OHT indicated and were kept without 4OHT for number of days
shown (+). (B,C,E-G) Back skin, (D) paw skin. Arrows in B and C indicate
epithelial outgrowths from the permanent portion of the hair follicles and
from sebaceous glands. Arrows in D indicate new hair buds arising from
interfollicular epidermis. (H-Q) expression of cyclin D1 (H), Lef1 (I,L),
keratin 17 (J,M), keratin 1 (K) and ß-catenin (green, N-Q; red
fluorescence is propidium iodide counterstain) in D2 tumours, after 28 days of
4OHT treatment (H-J,N,O) or 28 days of treatment followed by 28 (P,Q) or 106
days (K-M) without 4OHT. Arrows in I highlight Lef1 expression. O,P are high
magnification views of boxed regions in N, Q respectively. Scale bars: 100
µm (A-M), 50 µm (N-Q).
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