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Fig. 8. Tbx1–/– inner ear and VIIIth ganglion
morphology at E13.5. (A-D,F,G,J,K) Transverse sections through wild-type and
Tbx1–/– ears/ganglia at E13.5, reacted with
mAb2H3 (anti-neurofilament) and counter-stained with Cresyl Violet. Axes in F
apply to all except G. Red arrows in A and C indicate regions of vestibular
(vest) neuronal cytology and cyan arrows in C auditory (aud) neuronal
cytology. The distinguishing cytological features (nuclear size and
basophilia) from a mutant posterior ganglion are shown at high magnification
in G, with vestibular neuronal cytology shown at right. White asterisks in A
and B indicate a branch of the facial nerve. Section shown in F passes through
a Tbx1–/– posterior compound ganglion and
epithelial posterior pole. Pvest, presumptive vestibular ganglion; Paud,
presumptive auditory ganglion. Brackets and arrowheads in J and K indicate
cellular stratification. Scale bars: in D, 60 µm for A-D; 20 µm for J,K.
(E) 3-D reconstructions of serial sections reacted as described above.
Presumptive vestibular (red) and auditory (cyan) ganglion tissue is shown in
relation to the inner ear epithelium. c, cochlea; sa, superior ampulla; la,
lateral ampulla; pa, posterior ampulla; sc, superior canal; lc, lateral canal;
pc, posterior canal; ed/es, endolymphatic duct/sac; u, utricle; s, saccule;
lt, lateral tube; ms, medial sac; dp, dorsal projection. (H) Double
immunolabeling with mAb2H3 and anti-MATH1 shows neurites (in green,
arrowheads) enveloping the base of a MATH1-positive cell. Dotted line
indicates the basement membrane. (I) Anteroventrolateral view of the
Tbx1–/– inner ear; the region of
stratification and innervation is mapped in brown. Approximate section levels
are indicated. (L) MATH1-positive nuclei occupy an apical position in the
epithelium. Dashed line indicates the lumen, which is collapsed. Dotted line
indicates the basement membrane.
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