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Fig. 3. Representative immunolabeling of Ser9 phospho-GSK-3ß in the bow region
of cryosections from mouse lenses (A), counterstained with Hoechst dye (B).
Strong reactivity for phospho-GSK-3ß in fiber cells near the lens equator
(arrow), but not in lens epithelial cells (arrowhead). (C) Western blotting of
cell extracts was performed with anti-Ser9 phospo-GSK-3ß and normalized
with anti-total GSK-3ß. Ser9 phosphorylation of GSK-3ß was increased
in differentiating lens cells. Normalized quantification is shown in the
graph. (D) Protein extracts from undifferentiated lens epithelium (LE) and
differentiating lens fiber cells of the equatorial zone (FC) were assayed by
GSK-3ß immune complex kinase assay. GSK-3ß kinase activity was
decreased in the rat lens fiber cells. Each error bar represents the
mean±s.d. of three independent experiments; each assay was performed in
duplicate. le, lens epithelium; lf, lens fiber.
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