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Fig. 4. Localization of ß-catenin on mouse lenses. ß-catenin
immunostained cryosections of the bow region of mouse lenses. (A)
ß-catenin antibodies localized to the membrane of lens epithelial and
fiber cells. However, nuclear ß-catenin was detected only in
differentiating fiber cells near the lens equator (C,D). ß-catenin
antibodies did not label the nucleus of central (B) and equatorial (E)
epithelial cells. Scale bars: A, 50 µm; B-E, 10 µm. (F) Lysates prepared
from the nuclei of undifferentiated lens epithelial cells (lane LE) and
differentiating lens fiber cells (lane FC) were separated by 10% SDS-PAGE and
analyzed with antibodies against ß-catenin. Western blots showed that
nuclear ß-catenin was higher in differentiating lens cells compared with
undifferentiated epithelial cells. Antibodies against lamin A/C were used to
confirm the purity of the nuclear fraction.
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