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Fig. 5. Wnt CM induces elongation of lens epithelial cells. Explants were
pre-stimulated with 50 ng/ml FGF2 or Wnt3a CM for 1 hour, followed by
stimulation with control medium, Wnt3a CM, 50 ng/ml FGF for 5 days, or in the
presence of 50 µM U0126 or DMSO. Explants were also cultured with vehicle
or control medium in the absence of stimulation. Untreated explants or
explants cultured in control medium after pre-stimulation with FGF2 showed
normal epithelial cell morphology (A,C,E,I). Explants pre-stimulated by Wnt3a
and cultured in Wnt3a did not undergo morphological change (B). However,
explants that were pre-stimulated by FGF2 and cultured for 5 days in Wnt3a
elongated (F) similar to explants cultured continuously with FGF2 (D). In the
presence of DMSO (as a control for U0126), explants cultured with FGF2/Wnt CM
showed a cell elongation (G). However, cell elongation did not occur in the
presence of U0126 (H). Scale bar: 50 µm.
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