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Fig. 7. Wnt CM induces the accumulation of ß-catenin in nuclei. (A) Explants
were pre-stimulated by 50 ng/ml FGF2, followed by stimulation with control
medium or Wnt3a CM for 5 days. Explants were immunolabeled with
anti-ß-catenin or Hoechst 33258 to stain nuclei. ß-catenin
accumulated in the nuclei of cells that elongated after treatment with
FGF2/Wnt CM (b; arrow), but not in the nuclei of morphologically normal cells
cultured with control medium or FGF2/control medium (a). Scale bar: 10 µm.
(B) Nuclear extracts were prepared from explants cultured with FGF2/control
medium (F/C) or FGF2/Wnt CM (F/W). Western blotting was performed with
anti-ß-catenin. Explants displayed an increased level of nuclear
ß-catenin in response to FGF2/Wnt CM. Blots probed with an anti-lamin A/C
antibody demonstrated equal loading of nuclear proteins.
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