QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 9. Wnt CM induces alteration in the protein levels of cadherins and p57^Kip2 in epithelial cells pre-treated with FGF2. Explants were cultured for 5 days with control medium (lane F/C) or Wnt3a CM (lane F/W), after pre-stimulation with FGF2 for 1 hour. Lens epithelium (lane LE) and differentiating lens cells of the zone of early elongation at equator (lane FC) were isolated from neonatal rat lens. Cell lysates were separated by 10% SDS-PAGE and probed with antibodies against E-cadherin, N-cadherin, p27^Kip1 or p57^Kip2. E-cadherin levels were decreased and N-cadherin levels increased after treatment with FGF2/Wnt CM, consistent with the changes seen during fiber cell differentiation (A). p57^Kip2 levels increased in explants treated with FGF2/Wnt CM, similar to the increase in differentiating fiber cells. However, p27^Kip1 levels were not affected by FGF2/Wnt CM, unlike the expression pattern seen in differentiating fiber cells (B). Actin was used as a loading control.

Return to article