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Fig. 5. Formation of the IRS in Hair-Bmpr1a KO mice and control mice.
Frozen sections of hindlimb skin were prepared from control mice (A-D) and
Hair-Bmpr1a KO mice (E-H) at P14. Expression of keratin 6 (A,B,E,F)
in the IRS, and expression of keratin 5 (C,D,G,H) in the ORS were detected by
immunofluorescence staining. B,D,F and H are merged with Hoechst (blue) for
nuclear staining. Formation of IRS characterized by the expression of
IRS-keratin 6 was severely reduced in Hair-Bmpr1a KO mice (E,F).
(I,J) Ultrastructure of hair follicle in control mice (I) and
Hair-Bmpr1a KO mice (J) at P30. Insets show keratin 5 (red) and
Hoechst (blue) signals at P30. Absence of IRS cells resulted in proliferation
of keratin-5-positive ORS cells. c, cortex; ci, cuticle of IRS; he, Henle's
layer; hu, Huxley's layer; irs, inner root sheath; me, medulla; ors, outer
root sheath; th, trichohyalin granule. Scale bars: 100 µm in A-H and insets
in I,J; 5 µm in I,J.
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