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Fig. 3. Glomerular abnormalities in endothelium-specific Pdgfb knockouts.
Hematoxylin/Erythrosin (H/E) staining (A-F) and 
-smooth muscle actin
immunostaining (G-J) of E18.5 kidney glomeruli of different genotypes. Normal
glomeruli containing a mesangial cell core (outlined by arrowheads, G) were
seen in wild-type (A) and control (C,E,G) kidneys, whereas the
Pdgfb–/– (B) and some of the lox/– (D,J)
glomeruli completely lacked mesangial cells. In these cases, the glomerular
capillary tufts become replaced by single dilated capillary loops. Most
lox/– glomeruli show a reduced mesangial core (outlined by arrowheads,
H), leading to a reduction in tuft complexity and dilation of the remaining
capillary loops (F,H). (K) Morphometric analysis of glomerular capillary
dilation; the relative distribution of capillary diameters in glomeruli of the
same genotype is shown. The capillary diameters of the lox/– animals
differ significantly from those of all other groups (P<0.005).
Note that the range of values, but not their distribution, is similar for
lox/– and –/– (k/o). There is no difference between controls
(sum of flox/+, flox/–, lox/+) and wild type (P>0.05).
Measured diameters have been corrected to the closest 5 µm to yield an
illustrative figure. Scale bar: 10 µm.
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