|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Polycythemia is secondary to Epo-driven, bone marrow-derived red cell
production. (A) Real-time RT-PCR reveals elevated Epo transcript
levels in mutant liver and kidney at 3 weeks of age. The horizontal line
indicates the median. *P<0.05; **P<0.01;
***P<0.001. (B) In vitro, FACS-based determination of
late-progenitor and precursor erythroid cell differentiation to
Ter119-positivity. Pcm/Pcm mice at 3 weeks of age show increased bone
marrow erythroid activity at day 2. *P<0.001. (C) Increased
hemoglobinization and cellularity of Pcm/+ bone marrow, as
demonstrated by O-D staining of sections at 7 weeks of age. (D) In vitro
analysis reveals increased bone marrow erythroid activity in Pcm/+
mice at 7 weeks of age. *P<0.001. (E) Real-time RT-PCR using
12-week kidney samples shows no statistically significant differences in
Epo transcript levels among genotypes. The horizontal line indicates
the median.
|
