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Fig. 5. Bam acts redundantly with Dpp signaling components to downregulate Dpp signal transduction (anti-lacZ, green; anti-Hts, red). (A-C,G-J) Confocal projections of ovarioles from one- to two-day-old females; (D-F) three images from a single confocal section of a female of the same age. (A) A germarium of an ovariole from a bam^D86 P{lacZ}Dad^P1883/bam^D86 female showing that Dad-lacZ expression is confined to germ cells in the anterior tip of the germarium. (B) A germarium of ovariole from sax^B18 Tp(2;2)DTD48/+ +; P{lacZ}Dad^P1883/+ female. (C) A germarium of ovariole from Smurf^15C; bam^D86 P{lacZ}Dad^P1883/+ female. (D-G) Ovarioles from females of genotype sax^B18 Tp(2;2)DTD48/+ +; bam^D86 P{lacZ}Dad^P1883/bam^D86. (D-F) Three images from a single confocal section, illustrating the variability of the extent and intensity of Dad-lacZ expression: (D) posterior part of the ovariole with at least 20 germ cells that have high-level Dad-lacZ expression; (E) ovariole in which many germ cells express intermediate levels of Dad-lacZ; (F) ovariole with Dad-lacZ expression only in germ cells at anterior tip. (G) Expression of Dad-lacZ in germ cells scattered in salt-and-pepper fashion throughout ovariole. (H-J) Ovarioles from Smurf^15C; bam^D86 P{lacZ}Dad^P1883/bam^D86 females. (H) Upper ovariole, expression of Dad-lacZ throughout ovariole in germ cells adjacent to somatic cells. Lower ovariole, expression of Dad-lacZ in approximately five cells at anterior tip of germarium. (I) An ovariole containing at least 40 Dad-lacZ positive germ cells. (J) Expression of Dad-lacZ throughout ovariole in a salt-and-pepper fashion. In all panels, arrow indicates anterior tip of the germarium, brackets indicate germ cells expressing Dad-lacZ.

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