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Fig. 4. Real-time RT-PCR analysis of markers involved in the sex determination
process. Sox9 expression (shown on the X axis) in individual E13.5
knock-out gonads was variable with levels in some gonads being comparable to
those in female mice. (A) Mis expression was directly dependent on
Sox9 levels indicating direct activation of its promoter (correlation
coefficient r=0.9905; P<0.0001). Note that Mis
expression in mice with low levels of Sox9 was still higher than that
in control females. (B) Expression of Sox8 also showed a clear
dependence on Sox9 levels (r=0.8444; P<0.0001).
(C) No such direct relationship for the steroidogenic factor Sf1 was observed
(r=0.3173; P=0.2689). (D) Gonads with very low levels of
Sox9 showed persistent expression of the Sry gene.
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