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Fig. 9. Defects in testis formation in Sox9 heterozygous knock-out mice on a Sox8/Sox8 knock-out background. (A-C) Urogenital ridges isolated at E13.5 were analyzed for the presence of sex cords. Cords in Sox8/Sox8; Sox9{triangleup}/+ gonads were poorly outlined and the formation of the coelomic vessel was disturbed. (D-F) Immunofluorescent analysis of gonads at E15.5 (laminin, red; Mis, green). Sox8/Sox8; Sox9{triangleup}/+ gonads show a reduced number of sex cords, reduced expression of Mis and areas with poor differentiation (white arrowhead).





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