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Fig. 5. Robo misexpression shifts location of sensory arbors in the olfactory lobe.
(A-P) Orientation of the olfactory lobes is as described in the previous
figures. Relevant 1 µm confocal sections are stacked in each picture
(green). B,C,G,O are also labeled with the synaptic marker mAbnc82 (red). The
diagrams in D,H,L,P depict the positions of sensory terminals depicted from
several preparations. Colors indicate the anterior to posterior location of
the terminals as observed in confocal imaging of the lobe. Violet is most
anterior, followed by blue, green, yellow, orange with red appearing most
posteriorly. Arrows indicate the antennal commissure. Quantitative results are
presented in Table 2. (A-D)
lz-Gal4/+;UAS-GFP/+. Axons project to two glomeruli (arrowheads in A)
and cross over in the antennal commissure (arrow in A). Staining with mAbnc82
(B,C) allowed identification of these glomeruli as DM6 and DL3 (diagram in D).
(E-H) lz-Gal4/+;UAS-GFP/UAS-robo. Sensory terminals located centrally
within the lobe (arrowheads). (I-L) lz-Gal4/+;UAS-GFP;UAS-robo2.
Targets to only one lobe were frequently observed (arrowheads in I). (M-P)
lz-Gal4/+;UAS-GFP/UAS-robo3. Sensory neurons terminate at ectopic
positions and often show altered morphologies (arrowheads). (Q-V)
Cytoarchitecture of individual glomeruli. (Q,S) Visualization of
SG18.1-Gal4;UAS-GFP show that sensory arbors (green) are located in a
`doughnut-like' arrangement on the periphery of the glomerulus.
(Q',S') staining with the synaptic marker mAbnc82 (red) shows
presence of synapses within the outer region (yellow) as well as the core of
the glomerulus. (R,R'). GH146 UAS-GFP (green) shows position of
projection neurons within the glomerulus. These neurons arborize within the
entire glomerulus as can be seen with the overlap with mAbnc82 staining (red,
R'). (T-V) Three examples of ectopic glomeruli generated by
misexpression of Robo receptors show comparable organization of sensory
neurons and interneurons. The sensory neurons are located on the peripheries
(T,U,V) and the staining with mAbnc82 highlights both peripheral and central
regions of the glomerulus (T',U',V').
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