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Fig. 6. Impairment of tectal development in ntn mutant embryos. (A)
Confocal optical section images of the tectum of Bodipyceramide stained
wild-type (wt) and mutant (ntn) embryos at 28 hpf, 36 hpf and 40 hpf.
An arrow indicates cell-free spaces. Arrowheads point large and round cells
representing mitotically active cells. (B) TUNEL staining of coronal sections
through the tectum of wild-type (wt) and mutant (ntn) embryos at 30
hpf, 36 hpf and 40 hpf. An arrow indicates TUNEL-positive cells. (C) Confocal
composite images of immunostaining with anti-acetylated tubulin antibody of
wild-type (wt) and mutant (ntn) embryos at 30 hpf, 36 hpf and 40 hpf.
An arrow indicates the absence of tectal neuropil. (D) In situ hybridization
of brn3b mRNA in wild-type (wt) and mutant (ntn) embryos at
48 hpf. Dorsolateral view. Arrows indicate the tectum. Scale bars: 50 µm in
A,C; 100 µm in B,D.
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