|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Stabilization of the Hh target Ci is restricted, and Hh levels are reduced
in mutant clones. Hh dependent inhibition of Ci proteolysis appears as a band
of intense staining 8-10 cells wide anterior to Hh-expressing cells in the
posterior compartment. The remaining cells in the anterior compartment show
low levels of Ci staining. In ttv (A), sotv (B) or double
mutant clones (C), the domain of Ci stabilization is reduced to 1-2 cells in
width. Hh is uniformly distributed in the posterior compartment except in
clones lacking ttv (E), sotv (F) or ttv and
sotv (G). Reduced ligand levels are apparent in clones located along
the AP boundary (arrows) as well as deep within the posterior compartment
(arrowheads). (D,H) By contrast, Hh transcription, visualized using a Hh-lacZ
reporter (H), is unaffected in posterior clones mutant for sotv
(arrowhead). Mutant clones were visualized by the absence of GFP expression
(D).
|
