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Fig. 8. HS-derived disaccharides from sotv and ttv mutants. HPLC profiles of GAGs from wild-type, sotv and ttv larvae are shown (A). Peaks 1-6 represent the following disaccharides: ${triangleup}$ UA-GlcNAc, ${triangleup}$ UA-GlcNS, ${triangleup}$ UA-GlcNAc6S, ${triangleup}$ UA-GlcNS6S, ${triangleup}$ UA-2S-GlcNS and ${triangleup}$ UA2S-GlcNs6S, respectively. (B) A representative HPLC trace from sotv^18.2/Df(2R)Jp8 larvae at a six times more sensitive scale than in A. All six disaccharide species found in wild type can be detected in these animals, albeit at greatly reduced levels. (C) Disaccharides from sotv/Df(2R)Jp8 larvae are shown as a percentage of wild-type controls. The sotv alleles are ordered according to the levels of HS produced, which correlates with the predicted lengths of the mutant proteins (see Fig. 1). The results for ttv mutants are from two different experiments. In one case ttv^K06619 was derived maternally and in the other ttv⁰²⁰⁵⁵ was contributed maternally. Different colored bars indicate individual HS disaccharides.

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