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Fig. 9. HPLC profiles showing CS-derived disaccharides (A). The largest peak, migrating at $~$ 4 minutes retention time, represents unsulfated ${triangleup}$ Di-0S, followed by the ${triangleup}$ Di-4S disaccharide. Each tracing represents material from $~$ 40 larvae. (B) CS-derived disaccharides from sotv and ttv mutants, shown as a percentage of wild-type controls (fraction of ng disaccharide/mg dry tissue, Ore R control). Unsulfated ( ${triangleup}$ Di-0S) and monosulfated disaccharides ( ${triangleup}$ Di-4S) across the sotv allelic series show changes in total CS levels as well as the degree of sulfation.

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