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Fig. 3. PVF1 binds heparin. The capacity of PVF1 to bind heparin was examined as a
possible mechanism for activation of the ligand in the wing imaginal disc.
Schneider S2 cells were transfected with a PVF1-expression construct, and
medium was collected. Incubation of this medium with heparin beads showed that
most of the PVF1 protein was removed by the beads, and could be efficiently
eluted by 1.5 M NaCl. PVF1 did not bind to control sepharose beads. By
contrast, medium of cells expressing secreted GFP showed that the protein was
not bound to the heparin beads. Equal amounts of medium were loaded before and
after incubation with the beads. PVF1 was detected by probing the blot with
anti-PVF1 serum, and sGFP was followed with anti-GFP.
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