|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Clones lacking endogenous PVR have no apparent wing phenotype. (A,B) Clones
homozygous for a Pvr mutation (marked by absence of GFP) show normal
tissue organization and F-actin distribution in the larval wing imaginal disc.
Note that the size of the mutant clones is similar to that of the wild-type
twin clones showing enhanced levels of GFP. As the disc is slightly tilted,
this optical section shows the apical domain of the disc on the left part, and
the basolateral region on the right. (B) Note that normal F-actin accumulation
is observed in the former, and no ectopic accumulation in the latter,
regardless of the clone boundaries. (C-E) Similar Pvr mutant clones
were analyzed in the pupal wing, marked by the absence of ß-gal staining,
or PVR staining. In the case of PVR staining, only a background signal that is
not membrane-associated was detected within the clones, implying that the PVR
protein expressed by this allele does not contain the C-terminal domain
recognized by the antibody. Again, normal tissue organization and F-actin
distribution was observed.
|
