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Fig. 6. PVR overexpression induces actin polymerization. UAS-Pvr was
overexpressed in the wing disc by the dpp-Gal4 driver (A,D,F).
Elevation in actin microfilaments was observed in cells overexpressing PVR as
monitored by Phalloidin staining (B). Cross-section shows that the excess
actin filaments are located in the basolateral area of the cells (C, arrow).
Conversely, a reduction in actin monomers was observed in the same region by
staining with flourescein-DNaseI (D). Profilin (Chickadee), which binds free
actin and affects actin polymerization dynamics, is elevated in the stripe of
PVR overexpression (F, arrow). The changes in actin cytoskeleton following PVR
overexpression are eventually manifested in a general change in epithelial
morphology. An extra and irregular fold is observed and demonstrated with
FasIII which marks the outlines of all epithelial cells (H, arrowhead). E and
G show the domain overexpressing PVR.
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