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Fig. 2. hrb27C mutant germline clones display a dorsalized phenotype because of defects in Grk expression. (A) Wild-type egg with two dorsal appendages that mark the dorsal anterior surface. (B-D) hrb27C mutants lay a range of dorsalized eggs. For the two strongest alleles hrb27C^k02814 and hrb27C^rF680, the appendage defects observed range from being (B) widely spaced, with an expansion of the operculum in 28-62% of the eggs, to (D) a crown of appendage material that surrounds the anterior circumference of the egg in 5-6% of the eggs. More intermediate phenotypes were also observed (C) with a broad appendage that spans at least the width of the normal appendages in 31-60% of the eggs (n=86 for hrb27C^k02814 and n=262 for hrb27C^rF680). (E,F) grk in situ hybridizations of stage 9 egg chambers. In wild-type (E) grk is tightly localized to the dorsal anterior region of the oocyte, but is detected in a ring around the anterior circumference of a fraction of the egg chambers in hrb27C mutants (F). (G-I) Grk antibody staining of stage 9 egg chambers from hrb27C mutants reveals that Grk protein has a variable distribution that includes (G) tight localization to the dorsal anterior region as in wild-type, (H) diffuse localization throughout the oocyte with an enrichment in the dorsal anterior region, and (I) an anterior ring around the oocyte.

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