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Fig. 3. Hrb27C can bind to the 3'UTR of grk. (A) Schematics of the entire 3'UTR of grk, osk and nos with the regions used as probes or competitors underlined. (B) UV crosslinking analysis of ovarian proteins that can interact with grk, osk or nos. The banding profiles reveal that the same proteins bind to grk and osk RNAs, but these proteins do not bind to nos RNA. The identity of the protein that binds to grk and osk is Hrb27C because it can be immunoprecipitated with Hrb27C antibodies, but not with CycE antibodies. (C) UV crosslinking analysis of osk (labeled probe) in the presence of grk, osk (positive control) or nos (negative control) as unlabelled competitor. A 200-fold excess grk or osk is able to compete binding of Hrb27C (arrow) from osk, while excess nos is not.





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