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Fig. 4. Long-term silencing by PRE requires its continuous presence in the
transgene. X-gal stainings of wing imaginal discs from larvae that carry the
transgenes indicated on the left. Larvae were either not heat-shocked (no hs)
or were heat-shocked for 1 hour to induce flp expression and then allowed to
develop for the indicated number of hours before analysis (hs + xy hours).
Note the loss of expression after flp-mediated excision of the PRE
fragment during larval development. (Bottom) Time-course experiment; no X-gal
signal is detected 8 hours after flp induction but robust X-gal staining is
observed 12 hours after flp induction. Note that flp-mediated excision only
occurs in a fraction of the cells, and that the number of X-gal positive cells
increases over time due to cell division.
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