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Fig. 2. Grafted quail neural crest contribution to E15 chimeric corneas. All corneas are pigmented on the periphery (A,E,I,M). In addition, cardiac neural crest chimera corneas develop a darkly pigmented mass on their dorsal surfaces (E,I), which in some cases, grows feather buds (I, arrowhead). Cross-sections through representative corneas showing QCPN-positive (red nuclei) and MF20-positive (green) cells counterstained with DAPI. Midbrain control sections (B-D) show QCPN-positive cells in the corneal epithelium (Ep), stroma (st), endothelial cell layer (En) and ciliary muscle (Cm). Cardiac neural crest chimera corneas show numerous QCPN-positive cells and melanocytes in the ectopic mass (F-H), but fewer QCPN-positive cells in the layers of the cornea (J-L). All QCPN-positive cells in trunk neural crest chimera corneas (including those in the ciliary muscle, arrow) are pigmented.





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