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Fig. 1. Her5 activity at the midbrain-hindbrain boundary and nomenclature. All top
views (A-C) are flat-mounted embryos at the three-somite stage, dorsal views
with anterior upwards, revealed by in situ hybridisation for expression of the
genes indicated (colour-coded, left corner) (see also
Geling et al., 2003). Bottom
panels (A'-C') are interpretative drawings of the embryos in A-C
to introduce the specific nomenclature used in this work. At the three-somite
stage, her5 expression (A) encompasses most of the presumptive MH
(Tallafuss and Bally-Cuif,
2003) and separates the first ngn1-positive clusters (B)
within the anterior neural plate. These are the ventrocaudal cluster (vcc),
located in the basal diencephalon and anterior midbrain, the presumptive
motoneurons (r2M) and lateral neuronal precursors (r2L) in rhombomere 2. The
non-neurogenic domain identified by her5 positivity and ngn1
negativity around the MHB is called intervening zone (IZ) (white arrow in
B,B'). (C) Upon blocking Her5 activity by injection of a her5
morpholino (MOher5) into wild-type embryos, the medial (future
basal) part of the IZ domain is bridged by ectopic ngn1-positive
cells (blue arrow and blue box in C, compare with B). Thus, the IZ is composed
of a medial domain (red in B', absent in C', blue box in C) that
crucially requires Her5, and of a lateral domain (green in B' and
C') that exhibits additional blocks towards neurogenesis. Interpreted
from (Geling et al., 2003).
Hind, presumptive hindbrain; MH, mid-hindbrain domain; Pros, presumptive
prosencephalon; r4M, motorneurons of rhombomere 4; r4L, lateral neuronal
precursors in r4.
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