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Fig. 2. Hierarchical cluster of female and male somatic genes. Genes were identified by statistical analysis, clustered hierarchically using Cluster and visualized with Treeview (Eisen et al., 1998). Compromised ESTs (chimeric or from a contaminated source) were removed for this analysis (see Table 2). The cluster includes whole animal data from wild-type animals at all stages of Drosophila development, including embryos (E), larva (L), pupae (P) and adults (A) (Arbeitman et al., 2002), and from the following mutants: tud female (FTud) and male (MTud); XX tra (tra); XX Dsx^D pseudomales (dsxD); fru; and dsx intersexual XY (dsxXY) and XX (dsxXX). Expression relative to the reference RNA is shown; yellow, blue and black indicate high, low and median levels of expression, respectively (see scale bar). Results of RNA blot analyses are shown (RNA blot), with key below. Gene names are shown on the right; red indicates genes chosen for further analysis.

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