|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Temporal and tissue-specific expression in female internal genitalia. (A)
Schematic of the internal female reproductive system. Modified, with
permission, from Miller (Miller,
1950). (B) Expression profiles of two female genes (see
Fig. 2 legend). (C, parts I-IV)
In situ hybridization of wild-type and tud 5-day adult female
reproductive tissues using probes derived from female (I,II) and male (III,IV)
genes. I part a, II part a, and III parts a, h and i, are frozen tissue
frontal sections of whole animals; all other images are from whole-mount in
situ hybridization analysis. (I) Expression is seen in spermathecae (arrows)
and parovaria (arrowheads) in wild-type (a-d) and tud (e) tissues.
(II) Expression in nurse cells of stage 8-10 egg chambers (a-c); in follicle
cells covering an early stage 14 egg chamber, including the dorsal appendage
(arrowhead; inset is at a closer focal plane; d); in oviducts (e); in
spermathecae of wild-type (f) and tud (g) tissues; and in sheath of
the immature ovaries found in tud animals (g). (III) Expression of
male-enriched mfas in female tissues (a-f): in follicle cells of
stage 9-10 egg chambers (b,c); in sloughed off follicle cells of a later stage
egg chamber (d); in parovaria (e); in fat tissue (f); and,
sex-nonspecifically, in CNS (g,h). (IV) Expression of male-enriched
prd in female fat tissue (a-c). Insets are digital magnifications
unless otherwise noted.
|
