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Fig. 5. Temporal and tissue-specific expression in male internal genitalia (A)
Schematic of the internal male reproductive system. Modified, with permission,
from Miller (Miller, 1950).
(B) Expression profiles of male genes (see
Fig. 2 legend) (C) In situ
hybridization of wild-type 5-day-old (I-VI), or 0-24 hour (VII), adult and
mutant tud male reproductive tissues using probes derived from male
genes. I-V part a, VII part a, and II parts e and f are frozen tissue frontal
sections of whole animals; all other images are of whole-mount tissues. (I)
Expression in the accessory glands: CG18284 (a-c), CG17022 (d) and CG17843
(e). (II) Expression of CG8708 in the anterior ejaculatory duct (a-d; c and d
are different focal planes), including the papilla (d), and,
sex-nonspecifically, in adult and embryonic salivary glands (e,f). (III)
Expression of CG2858 in the ejaculatory bulb (a-e), showing individual
differences in expression (c-e). (IV) Expression in accessory glands and the
ejaculatory bulb: CG12558 (a-d) and CG9519 (e). (V) mfas expression
in testes (a-c) and on the surface of seminal vesicles where the testes attach
(c); in accessory glands (a,b,d); and in the ejaculatory bulb (b,e). (VI)
prd expression in testes (T) and accessory glands (A). (VII)
Expression of CG6788 in 0-24 hour ejaculatory bulb (a-c). Insets are digital
magnifications.
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