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Fig. 7. Expression of CG17012 and prd in wild-type and mutant tissues from
5-day-old flies. (A-D) Whole-mount in situ analysis of CG17012 expression in
female internal genitalia in: (A) wild type, (B) dsx intersexual
animals, (C) virgin wild type, and (D) tud females. In A and B, the
region within the box has been digitally magnified and shown on the right.
Staining is observed in cells of the spermathecae and in the fat cells that
surround the spermathecae in A, but only in the fat cells in B. Fat tissue is
not visible in C and D. (E) CG17012 sense probe (negative control). Low level
signal was observed in fat tissue, a common occurrence with both sense and
anti-sense probes. Arrows indicate cells of the spermathecae and arrowheads
indicate fat cells. (F) Whole animal frozen section in situ analysis of
prd expression in dsx intersexual and wild-type animals.
Arrowheads indicate expression regions
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