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Fig. 1. Fas2, Dlg and Slbo expression during oogenesis. (A) Transmembrane Fas2 binds to PDZ1 and PDZ2 domains of cortical Dlg. (B) Dlg is expressed in egg chamber germ cells (GCs) and follicle cells throughout oogenesis (g, germarium; s, stage of oogenesis; e, early; l, late). Border cell (BC) differentiation at stage 8 is marked by Slbo expression. At stage 9, the BCs migrate between the nurse cells (NCs) to the front edge of the oocyte (OO) (arrow). (C) Same egg chambers as (B). Fas2 is expressed in all follicle cells through stage 7 (see also D'). At stage 8, Fas2 expression is lost in anterior follicle cells that include the BCs (bracket), precisely at the time when the BCs differentiate. (D-D'') At stage 7, just preceding BC differentiation, Fas2 and Dlg are uniformly distributed around the circumference of the polar cells (PCs, inset). Fas2 and Dlg predominantly colocalize in all follicle cells (arrowheads), but at late stage 7, Fas2 starts becoming localized more apically (completes apical localization at stage 8, see E'), while Dlg remains uniformly distributed on the lateral membrane. (E-E''') Anterior of a stage 8 egg chamber. (E) When the BCs differentiate (Slbo expression), Dlg redistributes in the PCs toward the leading edge of the cluster (inset, arrows). (E') Fas2 expression is lost in stretch cells and BCs, but continues in PCs at the center of the cluster, where it becomes precisely colocalized with Dlg in a graded leading-to-trailing pattern (inset, arrows). BC extensions that express Dlg on their surface encapsulate the PCs at the leading edge of the cluster (E''', arrowheads). These extensions pioneer BCs invasion at stage 9 (F,F', arrow). Fas2 is expressed in PC, but not BC membranes (F', inset, arrows). (G) Fas2 and Dlg (inset) continue to be polarized in PCs toward the leading edge of the BCs as the cluster migrates (arrows). (H,I) Fas2 enhancer-trap expression during oogenesis. The Fas2 transcription pattern (H) resembles Fas2 protein pattern (C). At stage 9, Fas2 is expressed in PCs but not BCs (I).





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