(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Otx2flox inactivation by En1-driven Cre
recombinase. (A-N) En1cre/+ and
En1cre/+; Otx2flox/flox embryos are
hybridised at E9.5 and E10.5 with Otx2
(A,B,E,H,I,L) and
Cre (C,F,J,M) probes, or processed for Otx2 immunodetection
(D,G,K,N). The arrows in A,D,H,K indicate the approximate position of frontal
sections; the arrowheads in D,K indicate the ventral midbrain where Otx2 is
lost. Fb, forebrain; Mb, midbrain; Hb, hindbrain; rp, roof plate; fp, floor
plate.