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Fig. 7. Abnormalities in extent, identity and fate of progenitor domains. (A-F) In
situ hybridisation (A-E) and immunohistochemistry (F) on whole-mount embryos
focussing on sagittal view of the ventral midbrain of
En1cre/+ and En1cre/+;
Otx2flox/flox embryos at E12.5 and E11.2 with
Fgf8 (A), Pou4f1 (B), Th (C), Isl1 (D),
Pet1 (E) probes and 2H3 (F) antibody. (G-ß) Immunohistochemistry
(G-R,W-ß) and in situ hybridisation (S-V) at E12.5 in the midbrain of
En1cre/+, En1cre/+;
Otx2flox/flox and En1cre/+;
Otx2flox/flox; Otx1-/- embryos and in
the rostral hindbrain (M-R) of En1cre/+ embryos with
Nkx6.1 (G,M,W), Nkx2.2 (H,N,X), Pou4f1 (I,O,Y), Th (J,P,Z), Isl1 (K,Q,
)
and 5-HT (L,R,ß) antibodies and with Otx2
(S),
Fgf8 (T), Th (U) and Sert (V) probes. (
)
Schematic representation summarising the expression pattern of Shh, Nkx6.1 and
Nkx2.2 and neuronal cell types detected in control and mutant embryos. The
broken lines in A indicate the approximate position of frontal (G-L) and
horizontal (S-V, M-R) sections; the position of frontal sections of the triple
mutant (W-ß) are at a level similar to that of the conditional mutant,
but in the triple mutant this position is posterior to Fgf8
expression; the arrowheads in B-E and the arrows in S,U,V indicate the
corresponding position of Fgf8 expression and the arrows in T
indicate the posterior border of functional Otx2 domain; the inset in
H,N,X highlights the ventral expression of Nkx2.2; the bracket demarcates the
neuroepithelium expressing Nkx2.2 and generating Ser neurons in the midbrain
of mutant embryos (H,L,X,ß) and in the rostral hindbrain of control
embryos (N,R). OM oculomotor nucleus; RN, red nucleus; Fb, forebrain; Mb,
midbrain; Hb, hindbrain; TN, trochlear nucleus; OMn, oculomotor nerve; e, eye;
DA, dopaminergic cells; Ser, serotonergic cells.
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