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Fig. 8. Abnormal differentiation is maintained at later stages in the ventral midbrain of conditional mutants. (A-H) Immunohistochemistry in sagittal (A-D) and frontal (E-H) sections of E15.5 control and conditional mutant embryos with Pou4f1 (A,E), Th (B,F), Isl1 (C,G) and 5-HT (D,H) antibodies. The arrowheads in A indicate the position of frontal sections and the arrow in G indicates the residual Isl1-positive neurons. RN, red nucleus; SN, substantia nigra; VTA, ventral tegmental area; MHB, midbrain-hindbrain boundary; TN, trochlear nucleus; OM, oculomotor nucleus.





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