spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Cloning of lin-46 and its homology to MoeA and gephyrin. (A) Regions of the genetic and physical maps of linkage group V of C. elegans depicting the location of lin-46. Two cosmids shown rescued a lin-46 mutant. One of these was subcloned to yield the minimal rescuing BglI-NgoMI restriction fragment. The gene structure was determined by RT-PCR and sequence analysis. (B) An alignment of the predicted amino acid sequences of LIN-46 of C. elegans and C. briggsae. Black, identical residues; gray, similar residues. Vertical lines indicate exon boundaries. Arrows indicate positions of lesions in the mutant alleles: ma164, ma174 and ve1. Dots and asterisks indicate residues partially and highly conserved among gephyrin/MoeA homologues, respectively. (C) Schematics of LIN-46 related proteins: gephyrin, mammals; Cinnamon, Drosophila; CNX1, Arabidopsis; MoeA and MogA, E. coli; MOC-1, MOC-2 and LIN-46, C. elegans. MoeA-like (E) and MogA-like (G) regions are indicated.





Right arrow Return to article