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Fig. 4. Expression of lin-46:GFP in hypodermal cells during larval development. Fluorescence and DIC micrographs of animals bearing lin-46:GFP transgenes. Transgene arrays include a rol-6 marker that causes twists in the body. (A) L2 animal expressing lin-46:GFP transcriptional fusion showing fluorescent lateral hypodermal seam cells. (B) An L4-stage animal bearing the transcriptional fusion showing fluorescent seam cells apparently undergoing fusion. (C) L2 animals expressing a lin-46:GFP translational fusion. Fluorescence is seen in seam cells as well as two neurons (see text). The exposure time is three to eight times longer than for animals expressing transcriptional fusion. (D) Higher magnification showing nuclear and cytoplasmic localization of LIN-46:GFP in an L2 animal expressing the translational fusion. Pairs of seam cells often show similar fluorescence intensity. Scale bars: in A, 100 µm for A-C; in D, 10 µm.





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