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Fig. 4. Wnt5a and Wnt7a are required for gland formation in
grafts grown into ovariectomized hosts. (A-F) Haematoxylin-Eosin staining of
grafts grown for 3 weeks in ovariectomized hosts (A-E) and cycling host (F).
Note the presence of glands (gl) in the control
Wnt5a+/– graft (A) and Lef1-/-
(D) but not in the Wnt5a-/- (B),
Wnt7a-/- (C) and Wnt7a/Lef1-/- (E),
nor the wild-type graft grown in a cycling host (F). Scale bar: 50 µm.
(G-J) Pattern of Wnt gene expression during gland formation in the wild-type
uterus at P15 (G-I, adjacent sections) and in a 3 week-old wild-type graft
(J). Lumen (lum) and smooth muscle (sm) are indicated. (G) Wnt7a is
expressed in the `invaginated' luminal epithelium but not in the glandular
epithelium (gl). In a section that passes through the site of invagination
(J), we observe a boundary of Wnt7a expression between the luminal
epithelium and the glandular epithelium. Wnt5a is expressed in the
stroma surrounding the glands (H). Low levels of Wnt5a expression are
also detected in the epithelium. Wnt4 expression is most abundant in
the subepithelial stroma (I). Scale bar: 20 µm.
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