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Fig. 5. Stromal Wnt5a expression is required for gland formation in the uterus. (A) Schema of the recombinant graft procedure. The mesenchymal sheath is separated from the epithelial tube by mild trypsin digestion and gentle mechanical manipulation. The mesenchyme (mes) from either wild-type or Wnt5a^-/- is recombined with wild-type or Wnt5a^-/- epithelium (epi) and grafted under the renal capsule of an adult host. (B-E) Haematoxylin-eosin staining of the recombinants. Glands (gl) form in the wild-type mes/wild-type epi (B) and wild-type mes/Wnt5a^-/- epi (D) but not in the Wnt5a^-/- mes/wild-type epi (C), or in the Wnt5a^-/- mes/Wnt5a^-/- epi (E). (F-H) Haematoxylin-Eosin staining of grafts (frozen sections) derived from the same Wnt5a^-/- individual infected at birth by the retroviral backbone (control, F), Wnt5a expressing retrovirus (G) and a Wnt4 expressing retrovirus (H). We observe that Wnt5a rescues the formation of glands whereas Wnt4 does not. Scale bar in D: 50 µm.

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