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Fig. 6. Wnt5a is required for the uterotrophic response and for
DES-mediated repression of Wnt7a. Neonate (P0) uterine horns from
control and Wnt5a-/- individuals were separated into two
pools of grafts that were grown in two ovariectomized hosts for 3 weeks. Each
host received control (two left-hand columns) and mutant (two right-hand
columns) grafts. For each experiment, one host was injected intraperitoneally
daily from day 18 to day 20 with DES resuspended in saline and one host was
injected with saline alone, as indicated. Hosts were sacrificed on day 21 and
the grafts harvested for analyses. Results are shown for a
Wnt5a+/– individual and a
Wnt5a-/- individual. (A-H) Haematoxylin-Eosin staining at
low magnification (A-D; scale bar: 250 µm) and high magnification (E-H;
scale bar: 20 µm). Note the aberrant uterotrophic response in the
Wnt5a-/- graft (D) showing enlarged lumen and thin uterine
walls when compared with the Wnt5a+/– graft (B). The
Wnt5a-/- epithelium does show an increase in height and
thickness in response to DES (H). (I-T) In situ hybridization for
Wnt7a (I-L), Hoxa10 (M-P) and Hoxa11 (Q-T).
Wnt7a is repressed by DES in the Wnt5a+/–
control graft (J) but not in the mutant (L). Hoxa10 and
Hoxa11 are strongly repressed by DES in the subepithelial stroma of
the Wnt5a+/– control graft (N,R) but not in the
Wnt5a-/- graft (P,T). Lines delineate the limits between
the luminal epithelium, the stroma and the myometrium.
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