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Fig. 2. Ray 1 cells and the adult ray 1 are displaced anteriorly in
plx-1(ev724). The position of ray 1 cells was determined by
fluorescence microscopy using the ajm-1::GFP reporter in L3 males
(Baird et al., 1991;
Koppen et al., 2001). For all
panels, anterior is left and ventral is bottom. (A) Male rays develop from two
bilaterally symmetric ray/SET precursor cells (Rn cells, where
n=1-9). In the third larval stage (L3), ray 1-4 cell clusters (pink
arrow) lie ventral to their corresponding R1-4.p sister cells. The developing
hook (A; white arrow) is located ventral to the ray 1-2 cells. (B-D) The
position of adult male rays was determined by DIC microscopy. Ray 1 is
observed at an abnormal anterior position in plx-1(ev724) adult males
(C,D) when compared with wild-type males (B). In wild-type males, ray 1 (B;
white arrow) is observed in close opposition to ray 2 (B; black arrow). A mild
ray 1 anterior phenotype (ray 1 class 2) is scored when ray 1 (C; white arrow)
is observed just anterior to it normal position (C; black arrow), but is still
within the fan structure. A severe ray 1 anterior displacement phenotype (ray
1 class 1) is defined as a ray 1 located outside the fan area (D; white arrow)
even further anterior to ray 2 (D; black arrow). A ray 1 that is shorter than
in wild-type males is also characteristic of both types of anterior ray 1
displacement (B-D). Ray 1 cells (white arrow) are displaced anterior in
plx-1(ev724) L3 males (F) when compared with wild-type animals (E) of
the same stage. Other ray cells, including ray 2 cells (E,F; pink arrow), are
not affected in plx-1(ev724) L3 males. (G) A detachment of all rays
[ray 1 (R1) shown by large arrow] from the male tail syncitium (SET) is always
observed in adult wild-type males. (H) Anterior ray 1 (large arrow)
displacements in adult plx-1(ev724) males is usually accompanied by a
persistent adhesion to the SET. Scale bar: 25 µm.
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