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Fig. 3. Transcriptional and translational reporters for plexin 1 are
expressed in developing ray cells. For all panels anterior is left and ventral
is bottom. The expression pattern of GFP reporters for plx-1 (see
Materials and methods, schematics show constructs used) was determined in
transgenic males using fluorescence microscopy. A similar expression pattern
was observed in transgenic animals with an extra-chromosomal translational
reporter array (evEx168) or an integrated transcriptional reporter
array (evIs140). (A) Expression of evIs140 is observed in
the dividing Rn.a and Rn.p cells, but predominantly in R1.a and R1.p cells
(white arrows) in L3 males. (B) Expression of evEx168 encoding the
entire plx-1(+) coding sequence (minus four C-terminal amino acids,
see Material and methods) fused to a GFP reporter. Expression is observed at
the cell membrane of developing ray cells, predominantly in the 3-cell
clusters for rays 1 and 2 during the early L3 stage (white arrows). (C,D) Low
expression is detected in all ray precursor clusters at the late L3 stage
(white arrows). No expression is detected in adult rays for either the
transcriptional or the translational reporter (data not shown). Scale bars: A,
15 µm; B-D, 25 µm.
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