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Fig. 2. Sequence requirements for ACE3 function in amplification. (A) The BP
construct and ACE3 deletion mutants. For convenience, ACE3 (751 to 1071 in
Fig. 1) is numbered from 1 to
320. The shaded regions are conserved among four Drosophila species.
The 
region was identified by sequence analysis and has homology to the
ß region in ori-ß. The two Myb consensus binding sites are indicated
by stars with one of them outside the conserved shaded regions. The three
p120-binding regions are indicated by black bars, the sizes of which are not
to scale (Beall et al., 2002).
(B) Southern blot analysis of amplification levels for representative
independent transgenic lines. BP and ACE3 total deletion (AD) lines were
analyzed as controls in the same experiment. rDNA was used as the loading
control. (C) Quantitation of three independent assays for each independent
transgenic line according to the measurement of amplification levels in
Materials and methods. Average and standard deviation are presented in the bar
graph. The average fold amplification level is given below the name of the
construct. P values are presented for a comparison of each construct
to BP using unpaired, two-sided t-tests.
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