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Fig. 7. Anti-ORC2 antibody staining and BrdU labeling in follicle cells. All images were generated using the confocal microscope. Background was intentionally increased in images F, G, I and L to reveal presence or absence of faint staining patterns. (A) Wild-type with anti-ORC2. (B) The transgenic line for BP construct with anti-ORC2. (C) The transgenic line for Yes-3.8S construct with anti-ORC2. (D,E) The transgenic lines for pCaryos-3.8S construct with anti-ORC2, lines 1 and 3, respectively. Line 1 amplifies only to very low level due to genomic position effects, while line 3 amplifies to high level, as shown in Fig. 8. (F) k43^fs293/k43¹ with anti-ORC2. (G) satin with anti-ORC2. (H) chiffon null mutant, genotype chiffon^wf24 /Df(2)RA5, with anti-ORC2. (I) chiffon null mutant with anti-ORC2 as in H. (J) Wild type with BrdU labeling. (K) chiffon null mutant, genotype chiffon^wf24 /Df(2)RA5, with BrdU labeling. (L) chiffon null mutant, genotype chiffon^wf24 /Df(2)RA5, with BrdU labeling as in K. The circles indicate the nuclei.

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