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Fig. 8. Proteasome activity is reduced in the hlr mutant. GUS staining of
HS::AXR3 NT-GUS, a marker for the degradation of AUX/IAA proteins, in
wild type (A,C) and in the hlr-1 mutant (B,D). (A-D) 36-hour-old
seedlings stained for 0 (A,B) or 60 minutes (C,D) after the end of heat-shock
induction. (E) GUS activity in the absence or presence of the proteasome
inhibitor MG132. Relative activity was calculated as a percentage of the
number of seedlings with GUS staining in the roots per total number of
seedlings examined. Scale bar: 25 µm.
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