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Fig. 3. Embryonic expression pattern of Fkbp8. (A) Whole-mount in situ hybridization in E10 embryos. Fkbp8 appears uniformly expressed. Negative controls included the sense probe and Fkbp8-/- mutants hybridized with the antisense probe. The minimal signal in the mutant further indicated that the mutant Fkbp8 transcript is destabilized. (B) In situ hybridization of sections. Thoracic neural tube is on the right, and lumbar neural tube is on the left. No differences in expression could be detected in the neural tube along the dorsoventral or anteroposterior axis. (C) Immunofluorescence of neural tube sections from E9.5 wild type (+/+) and Fkbp8 mutant (–/–) embryos, stained with the FKBP8 antibody.





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